Applying the MYC-TAD-OCT4 fusion proteins in mouse iPSC generation leads to shorter reprogramming dynamics, with earlier activation of pluripotent markers in reprogrammed cells than wild type OCT4 (wt-OCT4). We utilized the highly potent transactivation domain (TAD) of MYC protein to engineer the human OCT4 fusion proteins. However the iPSC induction efficiency is still very low and with lengthy reprogramming process. The development of human induced pluripotent stem cells (iPSCs) holds great promise for regenerative medicine.
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